Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7070996

Title:Production of cultured human mast cells and basophils for high throughput small molecule drug discovery
Inventors:Rossi, Alexander B.; San Francisco, CA, USA
Summary:Herein is described a method of producing a proliferated population of CD34-negative progenitor cells, by contacting CD34-positive cells with flt-3 ligand and stem cell factor. The invention relates to methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells, such that a uniform population of cells proliferate. The size of the population is suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. Further disclosed are methods for screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. Methods of generating hematopoietic cells such as basophil cells are included.
Abstract:Provided are methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells. The methods generate uniform proliferated populations of cells. The proliferated populations contain a uniform population of a size suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. The invention includes screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. The invention also includes isolating a candidate bioactive agent that causes the altered phenotype. Additionally, cells formed according to the described methods are also encompassed by the invention.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7070996
Application Number:US2001000053355
Date Filed:08/11/2001
Date Published:04/07/2006
Assignee:Rigel Pharmaceuticals, Inc., South San Francisco, CA, USA


 
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