Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7078585

Title:Bacterial cell component-unresponsive model mouse
Inventors:Akira, Shizuo; Mino, Japan
Takeuchi, Osamu; Mino, Japan
Takeda, Kiyoshi; Mino, Japan
Summary:This invention describes a toll-like receptor knockout animal that is unresponsive to bacterial cell components, namely, peptidoglycan and a lipoprotein/lipopeptide. Further disclosed are methods for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. Also described are the means by which such a knockout mouse is generated, resulting in a homozygous disruption of the TLR2 gene in its genome, wherein such disruption results in no production of endogenous TLR2 protein, and wherein the resultant mouse exhibits the phenotype of being unresponsive to bacterial cell components such as a lipoprotein and lipopeptide. Applications are included in the screening of potential modifiers for a desired response in pharmaceutical use.
Abstract:A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene; the targeting vector is introduced into an embryonic stem cell; the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7078585
Application Number:US2000000889324
Date Filed:13/01/2000
Date Published:18/07/2006
Assignee:Japan Science and Technology Agency, Saitama, Japan


 
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