Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7081336

Title:Dual resonance energy transfer nucleic acid probes
Inventors:Bao, Gang; Mableton, GA, USA
Tsourkas, Andrew; Atlanta, GA, USA
Xu, Yangqing; Atlanta, GA, USA
Summary:This invention describes a composition for detecting target nucleic acid via dual nucleic acid probes with resonance energy transfer moieties. Described herein are fluorescent or luminescent resonance energy transfer moieties on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, such as mRNA, thereby generating an observable interaction. Further disclosed are lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize in a similar manner, thereby providing detectable signals for the rapid, specific and sensitive determination of hybridization in vivo. Applications are provided in the detection of nucleic acid target hybridization, as are therapeutic uses in assessing drug candidates, and in the genetic analysis of various diseases such as cancer.
Abstract:Dual nucleic acid probes with resonance energy transfer moieties are provided. In particular, fluorescent or luminescent resonance energy transfer moieties are provided on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, e.g. mRNA, to generate an observable interaction. The invention also provides lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize sufficiently near each other on a subject nucleic acid to generate an observable interaction. The invention thereby provides detectable signals for rapid, specific and sensitive hybridization determination in vivo. The probes are used in methods of detection of nucleic acid target hybridization for the identification and quantification of tissue and cell-specific gene expression levels, including response to external stimuli, such as drug candidates, and genetic variations associated with disease, such as cancer.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7081336
Application Number:US2002000179730
Date Filed:25/06/2002
Date Published:25/07/2006
Assignee:Georgia Tech Research Corporation, Atlanta, GA, USA


 
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