Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7083979

Title:Methods for enhanced retroviral-mediated gene transfer
Inventors:Williams, David A; Indianapolis, IN, USA
Patel, Vikram P; Germantown, MD, USA
Summary:This invention describes methods for enhanced virus-mediated DNA transfer using fibronectin (FN) or fragments thereof. Described herein are the means for increasing the efficiency of transduction of hematopoietic and other cells by retroviruses, such that the FN and FN fragments significantly enhance retroviral-mediated gene transfer into the cells, particularly into hematopoietic cells, including committed progenitors and primitive hematopoietic stem cells. Further disclosed herein are improved methods for somatic gene therapy which capitalize on enhanced transduction by a replication-defective recombinant retrovirus vector. Applications in gene therapy are included.
Abstract:A method to increase the efficiency of transduction of hematopoietic and other cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the cells, particularly hematopoietic cells including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on enhanced gene transfer, hematopoietic cellular populations, and novel constructs for enhancing retroviral-mediated DNA transfer into cells and their use.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7083979
Application Number:US1999000394867
Date Filed:13/09/1999
Date Published:01/08/2006
Assignee:Indiana University Foundation, Bloomington, IN, USA


 
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