Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7097833

Title:Selected cell delivery for heart failure
Inventors:Freyman, Toby; Watertown, MA, USA
Summary:This invention provides methods for delivering stem cells to a failing heart. Such means of delivery include increasing blood flow to tissue in a subject in need of such a treatment, as well as methods of regenerating tissue in the subject, methods of treating diseased tissue in the subject, methods of forming new blood vessels in tissue and new tissue such as myocardial tissue, methods of increasing angiogenesis in diseased tissue in a subject, and methods of preventing heart failure in a subject. Such methods comprise isolating autologous mononuclear cells from the subject, selecting from the isolated autologous mononuclear cells the lineage negative (Lin-) mononuclear cells, and transplanting locally into or adjacent to the tissue an effective amount of the Lin autologous mononuclear cells, which results in the formation of new blood vessels in the tissue and formation of new tissue. Specific therapies employing such tissue are further disclosed.
Abstract:This invention provides methods of increasing blood flow to tissue in a subject in need thereof, methods of regenerating tissue in a subject, methods of treating diseased tissue in a subject, methods of forming new blood vessels in tissue and new tissue, such as myocardial tissue, in a subject in need thereof, methods of increasing angiogenesis in diseased tissue in a subject, and methods of preventing heart failure in a subject, which methods comprise: a) isolating autologous mononuclear cells from the subject; b) selecting from the isolated autologous mononuclear cells of step (a) lineage negative (Lin) mononuclear cells; and c) transplanting locally into or adjacent to the tissue an effective amount of the Lin autologous mononuclear cells, resulting in formation of new blood vessels in the tissue and formation of new tissue. Additional methods provided for such uses further select from the isolated autologous mononuclear cells of step (a) lineage negative (Lin) mononuclear cells a subset of Lin mononuclear side population (SP) cells and transplant locally into or adjacent to the tissue an effective amount of the Lin SP cells. In other aspects, methods using tissue other than mononuclear cells for these uses are provided, which comprise (a) obtaining a cell suspension from the tissue or a second tissue of the subject; (b) selecting from the cell suspension step (a) side population (SP) cells; and (c) transplanting locally into or adjacent to the tissue an effective amount of the SP cells, resulting in formation of new blood vessels in the tissue and formation of new tissue.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7097833
Application Number:US2002000198101
Date Filed:19/07/2002
Date Published:29/08/2006
Assignee:Boston Scientific Scimed, Inc., Maple Grove, MN, USA


 
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