Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7101546

Title:In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development
Inventors:Tsang, Wen-Ghih; Sherman Oaks, CA, USA
Zheng, Tianli; Culver City, CA, USA
Wang, Yanping; Los Angeles, CA, USA
Summary:Described herein is a novel in situ method of providing insulin to a mammal. The invention relates to the discovery that there exists an intermediate, differentiated stage of pancreatic stem cells which can be matured in situ into a stable, expandable cell line that produces insulin in response to glucose. The method disclosed herein involves encapsulating a cell culture of propagating pancreatic cells with an alginate capsule that has been cross-linked with a divalent cation and enclosed with a polylysine membrane; the encapsulated cells are then inserted into the mammal, permitting the cells to mature into insulin secreting cells, and thereby avoiding the step of culturing the intermediate stage stem cells into later stage pancreatic cells.
Abstract:This invention relates to the discovery that an intermediate, differentiated stage of pancreatic stem cells exist that can be matured in situ into a stable cell line that produces insulin in response to glucose. These cells are advantageous in that they are both expandable and stable in culture. This invention avoids the step of culturing the intermediate stage stem cells into later stage pancreatic cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7101546
Application Number:US2002000326190
Date Filed:20/12/2002
Date Published:05/09/2006
Assignee:AmCyte, Inc., Santa Monica, CA, USA


 
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