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Stem Cell Related Patent Number US7105344
| Title: | Fluorescent proteins as cell-type specific reporters | | Inventors: | Hescheler, Jürgen; Köln, Germany | | Summary: | Described herein are novel embryonic stem cells transformed to express harmless fluorescent proteins. Related cell cultures, DNA constructs and transgenic animals are also disclosed. The invention relates to non-human mammalian embryonic stem (ES) cells stably transfected with a DNA construct comprising a DNA sequence coding for a non-cell damaging fluorescent protein. Further described is a cell-type-specific and a development-specific promoter operably linked with the DNA sequence, as well as a method for culturing such ES cells. Methods of toxicological testing and examination of substances using such cell cultures are included. Means are further provided for the use of such transgenic non-human mammals obtainable by such a method in the study of cellular development. | | Abstract: | The present invention related to non-human mammal embryonic stem (ES) cells stably transfected with a DNA construct comprising a DNA sequence coding for a non-cell damaging fluorescent protein and a cell- and/or development-dependent promoter operably linked with said DNA sequence; a method for preparing such ES cells; a cell culture obtainable by culturing said ES cells; a method for the toxicological examination of substances using such cell cultures; a method for producing transgenic non-human mammals using said ES cells; a transgenic non-human mammal obtainable by said method; and a method for examining stages of cellular development using cells of such a non-human mammal. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US7105344 | | Application Number: | US2002000084960 | | Date Filed: | 28/02/2002 | | Date Published: | 12/09/2006 | | Assignee: | Axiogenesis AG Germany |
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