| Title: | Methods of culturing embryonic stem cells and controlled differentiation |
| Inventors: | Pera, Martin Frederick; Prahran, Australia |
| Summary: | Described herein are methods of culturing undifferentiated embryonic stem (ES) cells via culturing of the ES cells in the presence of BMP-mediated default pathway antagonists. This invention provides a preparation of undifferentiated ES cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Further disclosed are means by which the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extra-embryonic lineage. Also provided are methods of culturing ES cells to improve stem cell maintenance and persistence in culture. The techniques disclosed herein include a culture of ES cells as well as differentiated cells derived from the embryonic cells that result from directed differentiation procedures. |
| Abstract: | The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention.
The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US7112437 |
| Application Number: | US2001000885679 |
| Date Filed: | 20/06/2001 |
| Date Published: | 26/09/2006 |
| Assignee: | ES Cell International Pte. Ltd., Singapore |
