Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7112576

Title:Compositions and methods for cryopreservation of peripheral blood lymphocytes
Inventors:Hubel, Allison; St. Paul, MN, USA
Summary:This invention comprises a novel composition and method for cryopreserving cells in a non-toxic medium, which is composed of the following: a balanced electrolyte solution, a cryoprotective agent that is arabinogalactan, or a biological or functional equivalent thereof, in an amount of 1% w/v to 40% w/v, a cryoprotective agent that penetrates the cell membrane, and freshly isolated lymphocytes, hematopoietic stem cells, lymphocytes which are modified ex vivo, or a combination thereof. The medium does not comprise dimethylsulfoxide or serum; the arabinogalactan, a biological or functional equivalent thereof, in the medium results in a high post-thaw survival rate for the freshly isolated lymphocytes, hematopoietic stem cells, or lymphocytes which are modified ex vivo.
Abstract:A composition and method for cryopreserving cells in a non-toxic medium is described. The composition and method provide cells for cell therapy and other in vivo applications.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7112576
Application Number:US1999000458862
Date Filed:10/12/1999
Date Published:26/09/2006
Assignee:Regents of the University of Minnesota, Minneapolis, MN, USA


 
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