Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7119248

Title:Antibodies against epitopes with homology to self antigens, methods of preparation and applications thereof
Inventors:Rajewsky, Klaus; Cologne, Germany
Mueller, Werner; Cologne, Germany
Roes, Juergen; Cologne, Germany
Summary:This invention describes methods of production of autologous monoclonal antibodies (AMABs), or antibody fragments, that bind an epitope of a mouse self-antigen. Described herein is the use a genetically engineered host animal that does not biosynthesize at least one epitope of the antigen and utilizes the lack of self-tolerance of the host to the epitope to produce antibodies specific to the antigen. Further disclosed are methods of isolating cells comprising the use of such AMABs that have specificity for a cell surface antigen. Also provided are methods of immortalizing an antibody-producing cell to produce a hybridoma capable of continuously secreting an antibody that binds the self-antigen epitope.
Abstract:This invention provides novel methods of obtaining autologous monoclonal antibodies (AMABs) to self-antigens or homologs thereof. The method involves obtaining a genetically engineered host animal that does not biosynthesize at least one epitope of the antigen and utilizes the lack of self-tolerance of the host to the epitope to produce antibodies specific to the antigen. The invention also encompasses the AMABs produced by the methods. The invention further encompasses methods of isolating cells comprising the use of such AMABs that have specificity for a cell surface antigen.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7119248
Application Number:US1994000226168
Date Filed:12/04/1994
Date Published:10/10/2006
Assignee:Miltenyi Biotec GmbH, Bergisch Gladach, Germany


 
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