Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US7144704

Title:Methods and reagents for preparing and using immunological agents specific for P-glycoprotein
Inventors:Mechetner, Eugene; Irvine, CA, USA
Fruehauf, John; Tustin, CA, USA
Summary:This invention describes immunological reagents and methods specific for a mammalian transmembrane protein having a non-specific efflux pump activity which has been established as being a component of multidrug resistance in cancer patients undergoing chemotherapy. Further described herein are methods for developing and using immunological reagents specific for certain mutant forms of this transmembrane protein, known as P-glycoprotein (Pgp), and for wild-type Pgp in a conformation associated with substrate binding or in the presence of ATP depleting agents. Also included are methods for screening a compound for the P-glycoprotein binding, which include incubation of a mammalian cell expressing P-glycoprotein in the presence or absence of a compound, followed by reacting of the mammalian cell with an immunological reagent specific for P-glycoprotein in a biochemical conformation adopted in the presence of a P-glycoprotein substrate, followed by a comparison of the binding of the immunological reagent to the cell in the presence of the compound with binding in the absence of the compound. Also provided are improved methods for identifying and characterizing anticancer compounds.
Abstract:This invention relates to immunological reagents and methods specific for a mammalian, transmembrane protein termed Pgp, having a non-specific efflux pump activity established in the art as being a component of clinically-important multidrug resistance in cancer patients undergoing chemotherapy. The invention provides methods for developing and using immunological reagents specific for certain mutant forms of Pgp and for wild-type Pgp in a conformation associated with substrate binding or in the presence of ATP depleting agents. The invention also provides improved methods for identifying and characterizing anticancer compounds.
US Patent Website:Click Here for Full Text of Patent
Title Number:US7144704
Application Number:US2002000114847
Date Filed:02/04/2002
Date Published:05/12/2006
Assignee:Oncotech, Inc., Irvine, CA, USA


 
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