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| Title: | Chimeric bird from embryonic stem cells |
| Inventors: | Etches, Robert J.; San Mateo, CA, USA Heyer, Babette; Menlo Park, CA, USA Diamond, Jennifer; Millbrae, CA, USA Mather, Christine; Millbrae, CA, USA Beemer, Kathleen; Pacifica, CA, USA Myers, Heather; Danville, CA, USA |
| Summary: | This invention introduces novel cultures of avian embryonic stem cells. Methods are described by which the avian embryonic stem cells are injected into recipient embryos to yield chimeras with a significant contribution of original phenotype from the original stem cell. Further disclosed are means by which transgene encoding exogenous proteins are stably integrated in the embryonic stem cells and are present in the somatic tissue of the resulting chimeras. Such transgenes may encode exogenous proteins expressed in endodermal, ectodermal, mesodermal, or extra embryonic tissue. The authors describe methods by which breeding the resulting chimera yields transgenic birds whose genome is comprised of exogenous DNA. |
| Abstract: | Sustained cultures of avian embryonic stem cells are provided. Injection of avian embryonic stem cells into recipient embryos yields chimeras with a significant contribution from the embryonic stem cell phenotype. Transgene encoding exogenous proteins are stably integrated in the embryonic stem cells and are present in the somatic tissue of the resulting chimeras. The transgenes may encode exogenous proteins expressed in endodermal, ectodermal, mesodermal, or extra embryonic tissue. Breeding the resulting chimera yields transgenic birds whose genome is comprised of exogenous DNA. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US7145057 |
| Application Number: | US2002000067148 |
| Date Filed: | 01/02/2002 |
| Date Published: | 15/12/2006 |
| Assignee: | Origen Therapeutics, Inc., Burlingame, CA, USA |