Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number USRE39006

Title:Method of detecting hematopoietic progenitor cells
Inventors:Houwen, Berend; Redlands, CA, USA
Tsujino, Yukio; Hyogo-ken, Japan
Morikawa, Takashi; Hyogo-ken, Japan
Ikeuchi, Yoshiro; Hyogo-ken, Japan
Hamaguchi, Yukio; Hyogo-ken, Japan
Wang, Fu-sheng; Hyogo, Japan
Hirai, Kojiro; Hyogo, Japan
Summary:This patent is a reissue of patent no. US5830701. Described herein is a method of counting hematopoietic progenitor cells using flow cytometry without the use of antibodies. Methods are described for the detection of immature cells, the construction of a cell distribution profile and the detection of sub-classes of hematopoietic progenitor cells. The invention relates to improved methods for detecting and/or counting the appearance of hematopoietic progenitor cells, which consists of a novel protocol for mixing a blood sample with a reagent that is capable of detecting immature cells, without employing immunological techniques. Information is gathered on the treated sample via the use of a particle analyzer and the constructed cell distribution profile, a delineated portion of which is characterized as a zone in which at least one subclass of hematopoietic progenitor cells appear, and the cells within that zone are counted. The claims of the invention include an improvement in simplicity and accuracy over previous detection methods.
Abstract:
US Patent Website:Click Here for Full Text of Patent
Title Number:USRE39006
Application Number:US2003000704463
Date Filed:07/11/2003
Date Published:07/03/2006
Assignee:Sysmex Corporation, Kobe, Japan


 
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